Morfometria do órgão vomeronasal em ratos adultos / Morphometric study of the vomeronasal organ in adult rats
AUTOR(ES)
Katerin Elena Bohoquez Grondona
DATA DE PUBLICAÇÃO
2005
RESUMO
The vomeronasal organ of 10 male and 10 female Wistar rats, adults, 120 days weighting between 210 and 318g, were used for this study to determine the histological structure and to verify the existence of sexual dimorphism. After perfusion, the organs were fixed by immersion in 2,5 glutaraldehyde in phosphate-buffered solution; isolated with the nasal septum, immersed in Perenyi solution for decalcification and then sectioned using classic morphometric and stereologic techniques. The sections qere placed on slides and stained with toluidina blue and analysed in light microscope. Diafanization of the organ was done and this sample was stained with acid hematoxilin. The constitution and distribution of the VNO tissue of male and female rats, sexually mature were described and quantified. We also established the vessel total area, total volume organ, the volume ratio occupied by the vessels in the total volume organ, total lumen area and total sensorial and no sensorial area. However there are no significative differences in the organ volume between male and female Wistar rats, the structures of this complex organ show remarkable differences.
ASSUNTO(S)
Órgão vomeronasal vomeronasal organ dimorphism morphometry rats dimorfismo ratos morfometria
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