Molecular identification of the agent of Q fever – Coxiella burnetii – in domestic animals in State of Rio de Janeiro, Brazil

AUTOR(ES)

Mares-Guia, Maria Angélica Monteiro de Mello, Rozental, Tatiana, Guterres, Alexandro, Gomes, Raphael, Almeida, Daniele Nunes de, Moreira, Namir Santos, Barreira, Jairo Dias, Favacho, Alexsandra Rodrigues, Santana, Andrea Lopes, Lemos, Elba Regina Sampaio de

FONTE

Rev. Soc. Bras. Med. Trop.

DATA DE PUBLICAÇÃO

2014-04

RESUMO

Introduction Over the last recent years, the number of Q fever cases have has increased throughout the world. An epidemiological investigation was performed in the area in which the first molecular documentation of Q fever in Brazil was previously reported. Methods Indirect immunofluorescence assay (IFA) and PCR of Coxiella burnetii targeting the htpAB gene were performed in samples from 14 dogs (blood); 1 cat (blood); 10 goats (blood, milk, vaginal swab and anal swab); 3 sheep (blood); and 2 horses (blood). Results Two dogs, two sheep and five goats were seroreactive. DNA was amplified from 6 milk and 2 blood samples from goats and from dogs, respectively. The sequence of the amplicons exhibited 99% sequence similarity with the homologous sequence of the htpAB gene of C. burnetii RSA 331 (GenBank - CP000890). Conclusions The results confirm C. burnetii infection in animals in Rio de Janeiro and reinforce the need for the surveillance of Q fever in Brazil.

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