Molecular characterization of Candida spp. isolates from patients with bloodstream infections

AUTOR(ES)

Xavier, Paula Cristhina Niz, Chang, Marilene Rodrigues, Paula, Claudete Rodrigues, Matsumoto, Flavia Emi, Asensi, Marise Dutra, Matos, Maria de Fatima Cepa, Andreotti, Renato

FONTE

Rev. Soc. Bras. Med. Trop.

DATA DE PUBLICAÇÃO

09/10/2013

RESUMO

Introduction The aim of this study was to conduct an epidemiological study comparing the genetic similarity of yeasts isolated from blood cultures. Methods Random amplification of polymorphic DNA (RAPD) techniques were used for the Candida samples obtained from patients at the Hospital Universitário da Universidade Federal do Mato Grosso do Sul (HU/UFMS) in Campo Grande, state of Mato Grosso do Sul, Brazil, from 1998-2000. Results The most frequently isolated species was Candida albicans (45.8%). DNA amplification from genomic yeast isolates indicated a genetic similarity of over 90%. Conclusions The RAPD profiles obtained were able to differentiate between the isolated Candida species, thereby suggesting that the method might be useful in epidemiological studies.

Documentos Relacionados

• Species distribution and antifungal susceptibility profile of Candida spp. bloodstream isolates from Latin American hospitals
• Trends in Antifungal Susceptibility of Candida spp. Isolated from Pediatric and Adult Patients with Bloodstream Infections: SENTRY Antimicrobial Surveillance Program, 1997 to 2000
• Biofilm Production by Isolates of Candida Species Recovered from Nonneutropenic Patients: Comparison of Bloodstream Isolates with Isolates from Other Sources
• Molecular Characterization and Identification of Biocontrol Isolates of Trichoderma spp.
• In vitro antifungal susceptibility of Candida spp. oral isolates from HIV-positive patients and control individuals