Leucocitos e inflamação em pacientes com anemia falciforme / Leukocytes and inflammation in sickle cell disease patients

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

Sickle cell disease patients suffer repeated episodes of vaso-occlusion. These episodes are characterized by ischemia-reperfusion injury and inflammation. Thus, sickle cell disease is considered a model of microvessel disease and a complex interaction among endothelial cells, platelets, erythrocytes and leukocytes. The aim of this study was to investigate the role of mononuclear leukocytes in the inflammatory process of sickle cell disease and the release of cytokines related to the NADPH oxidase components activation in mononuclear leukocytes from patients with sickle cell disease (SCD). TNF-a, IFN-y and IL-10 were detected in culture supernatants by standard commercial ELISA immunoassay kits. There was significant higher release of TNF-a by PBMC from healthy controls compared to SCD patients after 24h PHA stimulus (p-0.02) or 8h LPS stimulus (p=0.04). There was no significant difference in the release of TNF-a by monocytes from SCD patients compared to healthy controls. INF-y release by lymphocytes from SCD patients was significantly higher compared to healthy controls after 48h culture with PFfA (p=0.02). EL-10 release by SCD patients lymphocytes was higher than healthy controls in basal conditions (p=0.01), and after 24h (p=0.03) or 48h (p=0.01) of PHA stimulation. We conclude that the increased production of superoxide, gp9Vphai hyper-expression in monocytes from SCD patients, the increased release of IFN-γ by lymphocytes, can contribute to enhance inflammation in SCD patients.

ASSUNTO(S)

citokines citocinas anemia falciforme leucocytes inflamação leucocitos sickle cell disease inflammation

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