Isolation of the Self-Regulated Repressor Protein of the Hut Operons of Salmonella typhimurium
AUTOR(ES)
Hagen, David C.
RESUMO
In Salmonella typhimurium the structural genes of the enzymes responsible for histidine utilization (hut) are clustered in two adjacent operons. A single repressor regulates both operons. The repressor itself is a member of one of the hut operons and, thus, regulates its own synthesis. We have assayed the hut repressor by its ability to bind radioactive DNA to nitrocellulose filters. The binding is specific for DNA bearing the hut operons, and the binding is abolished by the inducer, urocanate. As a member of one of the hut operons, the repressor is inducible, subject to catabolite repression, and affected by a promoter mutation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=433364Documentos Relacionados
- Nature and Self-Regulated Synthesis of the Repressor of the hut Operons in Salmonella typhimurium
- Identification and characterization of a self-regulated repressor of translocation of the Tn3 element.
- The Evolution of Self-Regulated Transposition of Transposable Elements
- Transcription of the hut operons of Salmonella typhimurium.
- Analysis of Self-Regulated Learning at Each Level of Mathematical Creative Thinking Skill