Ishikawa, A. A. Development of analytical methodology for the investigation of methylenedioxy derivatives in samples of vitreous humor in fatal traffic accident. 2011. 67 f. Dissertation (Master) - Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, 2011. / Desenvolvimento de metodologia analítica para a investigação de metilenodioxi derivados em amostras de humor vítreo em vítimas fatais de acidentes de trânsito






The methylenedioxy derivatives are among synthetic drugs of illicit use most consumed in the occident, these are substances cause mental and motor disorders and has caused major threat to traffic safety, these effects affect the performance of drivers and may lead to traffic accidents. The vitreous humor is a biological matrix available in postmortem analysis, and has great relevance to forensic toxicology. It is a simple matrix, clean, easy collection and handling, good stability, highly recommended especially in cases of body injuries, partially carbonization or in a state of decomposition. This is because the vitreous humor is located in an anatomically isolated area, relatively protected from external contamination and invasion of microorganisms. The purpose of this study was to develop and validate an analytical methodology to detect and quantify methylenedioxy derivatives in samples of vitreous humor in fatal traffic accidents. To develop the method we used 1 mL of vitreous humor, liquid-liquid extraction with ethyl acetate solvent, derivatization heptafluorobutyric anhydride (HFBA) and detection by gas chromatography-mass spectrometry (GC/MS). The methodology was validated according to the parameters established by ANVISA and demonstrated linearity from 10 to 400 ng /mL. The limits of detection were between 1,0 ng/mL and 2,5 ng / mL. The accuracy had values between 97.1% -103.7%, the intra-assay precision was 4.54% -9.14% and inter assay precision between 6.92% -10.59%. The recovery was higher than 57.87%. The methodology validated was applied in vitreous humor of victims of fatal traffic accidents collected in the Centre of Forensic Medicine of Ribeirão Preto (CEMEL)


acidentes de trânsito. gc / ms gc/ms humor vítreo methylenedioxy derivatives metilenodioxi derivados traffic accidents vitreous humor

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