Influência de materiais odontológicos na capacidade de resposta de fibroblastos cultivados de polpa dental humana / Influence of dental materials on the response capability of cultured fibroblasts from human dental pulp

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

13/04/2012

RESUMO

The aim of the present study is to investigate the influence of dental materials (Single Bond, HEMA, Vitrebond, Ketac Molar e Dycal) in the inflammatory response of human dental pulp fibroblasts from permanent teeth in relation to inflammatory mediators expression. and production. Primary cultures were established from third molars pulp tissue. After the fourth passage, the fibroblasts were stimulated only by materials and also by the materials followed by LPS from E. coli for 6 and 24 hours. Data were statistically analyzed using Oneway ANOVA and Tukey post-test and Two-way ANOVA followed by Bonferroni post-test (p<0.05). SB10 (Single Bond 1: 100) and DY (Dycal) affected cell viability and consequently decreased cell metabolism. SB1 (Single Bond 1:1,000), SB10 (Single Bond 1:100) and VB (Vitrebond) followed by LPS E. coli decreased cell metabolism. Nitric oxide levels were reduced when fibroblasts were stimulated by KM (Ketac Molar). Pro-collagen type I expression was reduced when fibroblasts were stimulated by SB10 (Single Bond 1:100), SB (polymerized Single Bond) and DY (Dycal). SDF-1_/CXCL12 expression was increased for the group stimulated only by LPS from E. coli, SB10 (Single Bond 1:100) and DY (Dycal). IL-6 expression had a significant decrease in the group stimulated by H1000 (HEMA 1000 nM) and an increase for SB10 (Single Bond 1:100) group. The expression of IL-8/CXCL8 decreased when fibroblasts were stimulated by the three concentrations of HEMA and of Single Bond, VB (Vitrebond) and DY (Dycal) at 6 hours and increased for SB10 (Single Bond 1:100) and VB (Vitrebond) at 24 hours. There was decrease in SDF-1_/CXCL12 production for the three concentrations of HEMA and DY (Dycal) and a declining trend for the other materials tested. The production of IL-6 was increased by VB (Vitrebond) and KM (Ketac Molar). The production of IL-8/CXCL8 increased by SB1 (Single Bond 1:1,000), VB (Vitrebond) and KM (Ketac Molar) and decreased by SB10 (Single Bond 1:100) and DY (Dycal). Single Bond and HEMA, in different concentrations, decreased the production and the expression of molecules involved in the inflammatory process and, because of its cytotoxic, should be viewed with caution when in intimate contact with the pulp tissue. Calcium hydroxide caused intense cell death and did not stimulate the production of inflammatory mediators evaluated, but this event seems to be essential to the pulp tissue repair process and mineralized barrier formation. The glass ionomer cements used increased the production of chemokines related to the inflammatory process, therefore, these materials, although they have not caused death of many cells, must be used with restrictions.

ASSUNTO(S)

cell viability chemokine citocina cytokine dental materials fibroblast fibroblasto materiais dentários quimiocina viabilidade celular

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