Immobilization of Beauveria bassiana Lipase on Silica Gel by Physical Adsorption

AUTOR(ES)

Sugahara, Vanessa Hitomi, Varéa, Geni da Silva

FONTE

Braz. arch. biol. technol.

DATA DE PUBLICAÇÃO

2014-12

RESUMO

Extracellular lipase from Beauveria bassianastrain CG481 was immobilized by using thirteen different immobilization protocols. Silica gel was chosen as the most suitable adsorbent with 94.8% of activity yield. The adsorption on silica gel did not change the optimum pH (8.5) and temperature (45ºC) values of the free lipase (FL) for lipolytic activity, and it showed higher activities in extreme conditions (pH 9.0 to 10.5, 60ºC). The lipase immobilized on silica gel (ILS) showed enhanced stability at pH 7.0 after 120 h incubation (69.0%) when compared to FL (33.3%). The thermal stability was also enhanced by immobilization at 60ºC in aqueous (64.6%) and organic medium (95.1%), while FL showed only 40.6% of residual activity in aqueous medium and exhibited no activity for esterification reaction in n-heptane. The treatment of ILS with 0.8 M NaCl prevented lipase desorption while Triton X-100 (0.1%) resulted the enzyme leakage. The ILS was reused for four times for esterification reaction with 80.8% of initial activity.

Documentos Relacionados

• Determination of Atmospheric Benzene Concentration by Displacement following Adsorption on Silica Gel
• Adsorption of hidrogen peroxide on the surface of silica - titania mixed oxide obtained by the sol-gel processing method
• The novel mesoporous silica aerogel modified with protic ionic liquid for lipase immobilization
• The Influence of Physical and Chemical Factors on the Preparation of Silica Gel Media1
• ESTUDO DA IMOBILIZAÇÃO DE LIPASE EM SÍLICA OBTIDA PELA TÉCNICA SOL-GEL