IMMOBILIZATION AND RELEASE STUDY OF A RED ALGA EXTRACT IN HYDROGEL MEMBRANES / Estudo da incorporação e liberação de um extrato de algas vermelhas em membranas de hidrogel

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

In pharmaceutical technology hydrogel is the most used among the polymeric matrices due to its wide application and functionality, primarily in drug delivery system. In view of the large advance innovations in cosmetic products, both through the introduction of new active agents as the matrices used for its controlled release, the objective of this study was to evaluate the release and immobilization of a natural active agent, the ArctAlg® in hydrogel membranes to obtain a release device for cosmetics. ArctAlg® is an aqueous extract which has excellent anti-oxidant, lipolytic, anti-inflammatory and cytostimulant action. Study on mechanical and physical-chemical properties and biocompatibility in vitro of hydrogel membranes of poly(vinyl-2- pyrrolidone) (PVP) and poly(vinyl alcohol) (PVA) obtained by ionizing radiation crosslinking have been performed. The physical-chemical characterization of polymeric matrices was carried out by gel fraction and swelling tests and biocompatibility by in vitro test of cytotoxicity by using the technique of neutral red incorporation. In the gel fraction test, both the PVP and PVA hydrogel showed a high crosslinking degree. The PVP hydrogel showed a greater percentage of swelling in relation to PVA and the cytotoxicity test of the hydrogels showed non-toxicity effect. The cytostimulation property of ArctAlg® was verified by the cytostimulation test with rabbit skin cells, it was showed an increase at about 50% of the cells when in contact with 0,5% of active agent. The hydrogel membranes prepared with 3% of ArctAlg® were subjected to the release test in an incubator at 37°C and aliquots collected during the test were quantified by high performance liquid chromatography (HPLC). The results obtained in the kinetics of release showed that the PVP hydrogel membranes released about 50% of ArctAlg® incorporated and the PVA hydrogel membranes at about 30%. In the cytostimulation test of released ArctAlg®, the PVP device showed an increase at about 80% of cell population in relation of test control, showing to be the greater device to be used in processes of skin repair.

ASSUNTO(S)

hidrogel de pvp hidrogel de pvp hidrogel de pva liberação "in vitro". liberação "in vitro". hidrogel de pva

ACESSO AO ARTIGO

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