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Extraction and Quantitative HPLC Analysis of Coumarin in Hydroalcoholic Extracts of Mikania glomerata Spreng: ("guaco") Leaves

AUTOR(ES)
FONTE

Journal of the Brazilian Chemical Society

DATA DE PUBLICAÇÃO

2001

RESUMO

Methods for preparation of hydroalcoholic extracts of "guaco" (Mikania glomerata Spreng.) leaves were compared: maceration, maceration under sonication, infusion and supercritical fluid extraction. Evaluation of these methods showed that maceration under sonication had the best results, when considering the ratio extraction yield/extraction time. A high performance liquid chromatography (HPLC) procedure for the determination of coumarin in these hydroalcoholic extracts of "guaco" leaves is described. The HPLC method is shown to be sensitive and reproducible.

ASSUNTO(S)

mikania glomerata spreng (compositae) coumarin high performance liquid chromatography (hplc) extraction techniques




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