Estudo dos modos batelada e reciclo externo estacionário para a separação do ácido hialurônico por cromatografia de exclusão por tamanho / Study of batch and steady state recycling modes for hyaluronic acid separation by size exclusion chromatography
AUTOR(ES)
Anayla dos Santos Sousa
FONTE
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia
DATA DE PUBLICAÇÃO
23/09/2011
RESUMO
The hyaluronic acid (HA) is a linear polysaccharide with important applications in the pharmaceutical, medical and cosmetics industry. Its purification is necessary because it is in a mixture coming from a variety of sources, animal and non-animal. The aim of this work is the separation of HA obtained by fermentation in synthetic medium, using the technique of size exclusion chromatography. The best conditions for HA separation were determined using, initially, a batch operation size exclusion column. These conditions were maintained in all trials and served to obtain the parameters for the large scale process. The batch operation also evaluated the ion exchange chromatography technique coupled to size exclusion, in order to enhance the separation of HA by examining cationic and anionic resins. A comparative study was carried out between the batch process, with and without the inclusion of ion exchange, and the semi-batch process with steady state recycling (SSR), in order to obtain fractions of HA and proteins with high purity. The semi-batch process operated with SSR assessed in differents volumes of recycle (1.0, 1.5, 2.25 and 3.0 mL). The results obtained with the semi-batch process with SSR achieved a purification percentage between 92 % and 97% for HA, working with recycle volumes from 1.0 to 3.0 mL, showing to be feasible for HA, while for the proteins proved to be unfeasible, with a maximum of 60 % purity. The ion-exchange chromatography obtained good results with the use of cationic resin, with 98% of purity and a yield of 99 % for HA, unlike the anionic resin which exhibited a mass loss of HA around 50 %. The scale-up process for size exclusion chromatography proved to be possible, with similar results to batch operation
ASSUNTO(S)
cromatografia de troca iônica Ácido hialurônico biopolímeros cromatografia hyaluronic acid biopolymers chromatography ion exchange chromatography
ACESSO AO ARTIGO
http://libdigi.unicamp.br/document/?code=000824775Documentos Relacionados
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