Estudo comparativo da biocompatibilidade in vivo de matrizes acelulares de pericárdio bovino e porcino / In vivo biocompatibility study of acellular bovine and porcine pericardium matrices

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

01/02/2011

RESUMO

Acellular matrices are used in tissue engineering for reconstruction or replacement of damaged tissues .In this study acellular matrices were developed using porcine pericardium and bovine pericardium, which contain a large quantity of type I collagen, using an alkaline hydrolysis treatment at the times of 12 hours and 24 hours. The matrices were developed to be used as protective barriers in the process of wound healing, and also in bone tissue inducing formation. In the test of in vivo biocompatibility, matrices were implanted subcutaneously in 65 adult male Wistar rats, sacrificed at 7, 14, 21, 28 and 35 days postoperatively. The microscopic evaluation on day 7 showed a large amount of inflammatory infiltrate around the material; at the 14th day, beyond the inflammatory infiltrate was observed a neovascularization. At 21st and 28th days were observed a gradual decrease in the inflammatory infiltrate, cellular growth and a large neovascularization. By day 35, porcine and bovine pericardium matrices were completely resorbed. Comparing the degradation time between the matrices treated with 12 and 24 hours, it was observed that both matrices treated for 24 h, degraded faster than those treated for 12 hours. Both matrices are biocompatible, do not cause cytotoxic reactions, inflammatory or immune adverse reactions.

ASSUNTO(S)

biocompatibilidade biocompatibility bovine pericardium collagen matrices matrizes de colágeno pericárdio bovino pericárdio porcino porcine pericardium

ACESSO AO ARTIGO

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