Emprego do colageno com organização supramolecular na regeneração nervosa periferica in vitro e in vivo / Influence of a naturally supraorganized collagen on the peripheral nerve regeneration in vitro and in vivo

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

12/03/2010

RESUMO

The present study investigated in vitro and in vivo the influence of naturally supraorganized collagen on the peripheral nerve regeneration. In the in vitro study, Schwann cells (SCs) were seeded on naturally supraorganized collagen membranes and evaluated with immunocytochemistry and scanning electron microscopy. The results revealed that SCs kept the expression of basal lamina components and adjusted their morphology to populate the membrane surface. For the in vivo study, adult female Sprague Dawley rats were divided into 4 groups: 1) TP – animals that received an empty polyethylene tube (n=7); 2) TPCL – animals that received an empty polycaprolactone (PCL) tube (n=5); 3) TPCLF – animals in which a polycaprolactone (PCL) tube filled with a naturally supraorganized collagen fragment was used (n=7); 4) AE – animals in which the nerve repair was carried out with an autologous nerve graft (n=5). The in vivo results were examined by counting of myelinated axons, morphometrical analysis of regenerated fibers and transmission electron microscopy, 60 days after surgery. Moreover, the in vivo study included immunohistochemical analysis and polarization microscopy. In this case, the specimens were obtained from one of 3 groups: 1) normal – normal sciatic nerves (n=3); 2) TPCL – animals that received an empty polycaprolactone (PCL) tube (n=3); 3) TPCLF – animals in which the nerve repair was carried out with a polycaprolactone (PCL) tube filled with a naturally supraorganized collagen fragment (n=3). The counting of myelinated axons provided significantly higher values in the AE group as compared to the other groups. Also, TPCL and TPCLF groups displayed higher values when compared to TP group. Regarding the morphometrical analysis, myelin thickness was greater in TPCLF group, indicating a better SC response during the regeneration process. The data regarding transmission electron microscopy and polarization microscopy indicates that TPCLF group achieved a better reorganization of the regenerated nerve microenvironment, resembling the normal nerve morphology. The immunohistochemical results were reinforced by the anti-p75NTR labeling, that demonstrated more active SC in the TPCLF group. The present results indicate that the use of naturally supraorganized collagen positively influenced/stimulated peripheral nerve regeneration possibly by increasing the activity of SC inside the prosthesis.

ASSUNTO(S)

nervos perifericos - regeneração tubulização materiais biomedicos colageno celulas de schwann peripheral nerves tubulization biomedical materials collagen schwann cells

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