Efeito do acetato de chumbo associado ou não ao sulfato ferroso em cérebro de ratos: análise das enzimas antioxidantes / Effect of lead acetate with or without iron sulfate in brain of rats: Analysis of antioxidant enzymes
AUTOR(ES)
Maria Cecilia de Freitas Ferreira
FONTE
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia
DATA DE PUBLICAÇÃO
14/09/2012
RESUMO
Lead (Pb2+) is a toxic heavy metal, found in all stages of the inert environment and in several biological systems. When uptaken by the organism, acts on several organs and systems and may cause severe damage in Central Nervous System. In addition, it has been reported that iron (Fe2+) may present, a protective effect on neurotoxicity caused by Pb2+. Therefore, the aim of this study was to evaluate the markers of oxidative stress in the brain of rats exposed with lead acetate (Pb(C2H3O2)2) associated or not with ferrous sulfate (FeSO4). Thus, 36 rats weaning (Rattus norvegicus) were, divided into 6 groups (G) of six animals and were exposed for six weeks.In the control group (G1), the animals received deionized water; G2 and G3 received 0,26 mM, G4 and G5 1,05 mM of lead acetate; in addition to this G3 and G5 were supplemented with 20mg of ferrous sulfate/Kg body weight every 2 days; G6 received deionized water and ferrous sulfate were used. The animals\ brains were collected for analysis of the enzymatic activity of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), the concentration of reduced glutathione (GSH), lipid peroxidation (TBARS), lipid hydroperoxide (LH) and total antioxidant substances (TAS) (ABTS and DPPH technics). The activity of the enzymes SOD and GPx in the experimental groups decrease compared to control, as well as the concentration of GSH (p<0.05). Concerning to the analysis of HL and CAT, the first tended to increase the concentration in experimental groups without concomitant exposure with FeSO4, while the second showed a slight tendency for increase in activity compared to control (p>0.05). The dosage of TAS-ABTS showed an increase in the groups exposed with 1,05 mM of lead acetate. Regarding the SAT- DPPH there was a decrease in the experimental groups (p <0.05). According to the results, the enzymes SOD and GPx and GSH were affected by lead acetate and exposure with ferrous sulfate change this dynamic. However, further studies are needed to verify if ferrous sulfate acts as a protective against toxic effect of lead acetate.
ASSUNTO(S)
acetato de chumbo antioxidantes antioxidants brain cérebro ferrous sulfate lead acetate sulfato ferroso
Documentos Relacionados
- Estado de maturação do nervo auditivo e tronco encefálico em ratos expostos a acetato de chumbo e suplementados com sulfato ferroso
- Experimental osteoarthritis in rats: evaluation of antinociceptive and chondroprotective effects of glucosamine sulfate and chondroitin sulfate
- SuplementaÃÃo semanal com sulfato ferroso associado ou nÃo à Vitamina A em escolares: um estudo de base experimental
- Uso do Metilfenidato em ratos: Análise do parênquima pulmonar
- Reconstrução do ligamento cruzado cranial em cães, associado ou não ao sulfato de condroitina