DEVELOPMENT OF SEMI-SOLID PREPARATIONS CONTAINING EXTRACT OF Sphagneticola trilobata (L.) AND PRUSKI (Acmela brasiliensis, Wedelia paludosa) (ASTERACEAE), AND EVALUATION OF IN VIVO TOPICAL ANTIINFLAMMATORY ACTIVITY / DESENVOLVIMENTO DE PREPARAÇÕES SEMI-SÓLIDAS CONTENDO EXTRATO DE Sphagneticola trilobata (L.) Pruski (Acmela brasiliensis, Wedelia paludosa) (ASTERACEAE) E AVALIAÇÃO DA ATIVIDADE ANTIINFLAMATÓRIA TÓPICA IN VIVO

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

he development phytotherapeutic dosage forms requires a series of stages in order to obtain an efficient, safe, and high quality preparation. The Sphagneticola trilobata (L.) Pruski (Asteraceae) is a native plant of Brazil, which frequently grows in coastal regions and barren land. In Portuguese, it is popularly known as Pseudo-arnica, Margaridão, Picão da praia and Vedélia, and in English, Creeping ox-eye or Trailing daisy. Studies carried by NIQFAR researchers have shown that this plant presents antiinflammatory, analgesic and antidiabetic properties, and it is therefore a plant with potential application for the development of phytotherapies. The aim of this work is to develop semisolid preparations containing hydroalcoholic extract of S. trilobata, and to evaluate its in vivo antiinflammatory activity. The extracts were prepared by turbo-extraction using hydroalcoholic solution as solvent, and characterized by physical-chemical assays and chromatography (TLC). The solvent was evaporated to obtain concentrated extract. Emulsified pharmotechnic bases were developed (cream), with hydrophilic characteristics (gel and PEG) and hydrophobic characteristic (unguent), containing concentrated extract The preparations were submitted to previous stability studies (freeze-thaw cycle) and accelerated stability studies (storage at room temperature, fridge and oven). The anti-inflammatory activity was evaluated through the ear edema method, induced by croton oil with topic application of 0.5 g of the preparations (with or without extract). As positive control, a preparation containing 1 mg/g dexamethasone was applied. The irritation potential of the formula was determined through the in vitro method of diffusion in agar. The extracts presented organoleptic and physical-chemical characteristics, within the limits found in the extraction process standardization study. Of the formula developed, the gel proved to be physically unstable when the plant extract was added. After the previous stability tests, the cream, unguent and PEG preparations containing concentrated extract proved to be stable in relation to the physical-chemical and organoleptic characteristics, except for the PEG containing extract, which presented a non-characteristic odor. The products submitted to the accelerated stability tests proved to be stable during the 90 days of study, in different storage environments. In the in vivo anti-inflammatory activity, a significant dose-dependant inhibition was observed for all the preparations. The results demonstrate that semisolid preparations containing extract of S. trilobata possess antiinflammatory properties, without causing irritation, and therefore have therapeutic potential for use in popular medicine.

ASSUNTO(S)

asteraceae farmacia produtos naturais anti-inflammatory activity phytotherapy fitoterápico atividade antiinflamatória asteraceae sphagneticola trilobata (acmela brasiliensis, wedelia paludosa) sphagneticola trilobata (acmela brasiliensis, wedelia paludosa)

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