Desenvolvimento e validação de metodologia analítica para a determinação de canabidiol e tetraidrocanabinol em amostras de plasma por cromatografia em fase gasosa/espectrometria de massas / Development and validation of an analytical methodology for determination of cannabidiol and tetrahydrocanabinol in plasma samples using gas phase chromatografy /mass espectrometry

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Dissertation (Master). Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo, Ribeirão Preto, 2008. The Cannabidiol (CBD), that represents about 40% of the cannabinoids found in Cannabis sativa plant, is devoided of the typical psychological and cognitive effects of 9-Tetrahydrocannabinol (9THC). Researches suggest that CBD shows ansiolitic properties, but this substance was never tested on clinical anxiety. It is unknown how possible CBD effects could be centrally mediated in social anxiety disorder (SAD) patients. There are evidences about the existence of a cannabinoid system in humans and there is also increasing interest on therapeutic CBD application. The present study was conducted to develop and validate an analytical methodology for determination and quantification of CBD and 9THC, employing gas phase chromatography/mass spectrometry technique. The validated method was fast, easy, low cost, with a good sensibility and appropriate for clinical toxicology applications. The method was linear on the range of 5 to 500 ng/0,5 mL of plasma for CBD (r2=0,99) and 5 to 300 ng/0,5 mL (r2=0,98) for 9THC. The detection and quantification limits were 0,1 ng/0,5 mL and 0,5 ng/0,5 mL for CBD and 5ng/0,5 mL and 10 ng/0,5 mL for 9THC respectively. Inter and intraday reproducibility were between 5,5% to 12,7% and 2,1% to 8,1%, respectively. Inter and intraday accuracy was between 1,2% to 12% and 1,2% to 14,5% for CBD and 9THC respectively. The recovery of extraction was between 54, 6% to 93,2% of recovery for the analytes The validated methodology was applied in a clinical trial to correlate the doses after the controlled cannabidiol administration to patients with social anxiety disorders.

ASSUNTO(S)

9-tetraidrocanabinol mass spectrometry anxiety espectrometria de massas validação ansiedade 9-tetrahydrocannabinol cromatografia em fase gasosa gas chromatography canabidiol validation cannabidiol

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