Comparative study and histomorphometric analysis of bone allografts lyophilized and sterilized by autoclaving, gamma irradiation and ethylene oxide in rats
AUTOR(ES)
Almeida, Otavio Machado de, Jorgetti, Wanda, Oksman, Denis, Jorgetti, Camilo, Rocha, Diógenes Laércio, Gemperli, Rolf
FONTE
Acta Cir. Bras.
DATA DE PUBLICAÇÃO
2013-01
RESUMO
PURPOSE: To compare three sterilization methods (autoclave, gamma irradiation and ethylene oxide) over non demineralized lyophilized bone allografts. METHODS: Bone allografts were implanted on paravertebral muscles of 21 rats. After 30 days animals were sacrificed and grafts underwent comparative analysis regarding histomorphometric and macroscopic parameters. RESULTS: Allografts that underwent the three sterilization methods presents similar weight gain, cortical thickness similar to control group, and less fibrosis than the control group. Grafts that underwent sterilization in autoclave presented less presence of multinucleated giant cells, although not statistically significant. There was also no statistically significant difference regarding mineralization on the three groups. CONCLUSION: The three sterilization methods cause similar effects on bone allografts regarding macroscopic and histomorphometric parameters.
Documentos Relacionados
- Effects of alveolar bone displacement with segmental osteotomy: micro-CT and histomorphometric analysis in rats
- Comparison of Autoclave and Ethylene Oxide-Sterilized Membrane Filters Used in Water Quality Studies
- Selective COX-2 Inhibitor (Meloxicam) and Tooth-Supporting Bone Quality. A Histomorphometric Study in Rats
- Chromatographical profiles of fluid extracts and tinctures obtained from Mikania glomerata Sprengel sterilized by gamma ray irradiation
- Bone histomorphometric analysis in familial hypocalciuric hypercalcaemia.