Biomembrana de quitosana-alginato na cicatrização de úlceras cutâneas em ratos. / Chitosan-alginate biomembrane on wound healing in rats.
AUTOR(ES)
Guilherme Ferreira Caetano
FONTE
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia
DATA DE PUBLICAÇÃO
24/09/2012
RESUMO
Chronic wounds require a long treatment time, resulting in high-cost medical care. Therefore, the search for new natural therapeutic healing substances, non-toxic and easy access to population has gained importance in recent years. The purpose of this study was to evaluate the effectiveness of chitosan-alginate biomembrane on wound healing in rats. Two excisional wounds were performed by punch (1.5 cm diameter) on the dorsal of 40 male Wistar rats. Both wounds of each rat were treated with chitosan-alginate biomembrane (BQA group) and treated only with saline (SF group) for 2, 7, 14 and 21 days post-injury. All wounds received occlusive dressing with gauze and tape and the biomembranes were removed only in the sacrificed day. It was evaluated the re-epithelialization by wound healing rate and by histomorphometry was quantified the inflammatory cells, fibroblasts, blood vessels and collagenesis by ImageJ software. Myeloperoxidase assay (MPO) was performed to evaluate the neutrophilic infiltrate on injured tissue, and hydroxyproline assay (OHP) to evaluate the collagenesis. BQA increased the recruitment of inflammatory cells to wound, different from SF (p=0,0134) on 2nd day. On 7th day, regarding to MPO, BQA showed an important reduction relation to 2nd day (p=0,0326), whereas SF showed higher MPO than BQA (p=0,0043). BQA increased the recruitment of fibroblasts to wound on 7th (p=0,0275) and 14th (p=0,0086), showed higher collagenesis than SF on 2nd (p=0,0042) and 21st days (p=0,0249), however on 14th day SF was higher (p=0,0010). BQA showed more pronounced reepithelialization on 7th day than SF (p=0,0006). Chitosan-alginate biomembrane regulated the inflammatory phase and stimulated the collagenesis, accelerating the wound healing on rats.
ASSUNTO(S)
alginate alginatos chitosan cicatrização cutaneous ulcer healing mice quitosana ratos Úlcera cutânea
Documentos Relacionados
- Optimization of alginate-chitosan multiparticulate systems for colonic drug release
- Desenvolvimento de curativos de quitosana e alginato contendo fosfato hidrogenado de zircônio, sódio e prata
- Gel de quitosana à 2% na cicatrização de feridas cutâneas em ratas diabéticas
- Enhancement of cellular activity in hyperglycemic mice dermal wounds dressed with chitosan-alginate membranes
- Efeito do laser terapêutico na cicatrização tendinosa: estudo experimental em ratos.