Biofilm formation by atypical enteropathogenic Escherichia coli. / Formação de biofilme por Escherichia coli enteropatogênica atípica.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2010

RESUMO

The atypical Escherichia coli enteropathogenic (EPECa) are capable to cause the lesion A/E and they dont transport the pEAF. Microbial biofilms are defined as complex communities formed by microrganisms adhered to surfaces embedded in an exopolysacharidic matrix. The objective of this study was to verify the capacity of formation of biofilm of 92 strains of atypical EPEC in abiotics and pre-fixed cells surfaces, using three methodologies (counting of UFC/cm2), colorimetric assay with violet crystal and CLSM). The gene shf and bfpA seem not to have relationship with the biofilm formation. There was not significant difference of biofilm formation in the tested surfaces. Through CLSM it was possible to verify the biofilm formation in EPECa. The biofilms visualized in CLSM revealed a great heterogeneity of the strains. A qualitative methodology, like CLSM should be used to indicate the biofilm formation. The adhesion and biofilm formation for atypical EPEC of long period can be a possible explanation for the cases of persistent diarrhea.

ASSUNTO(S)

biotechnology escherichia coli diarrhea atypical epec epec atípica biofilms biotecnologia diarréia adhesion biofilmes escherichia coli adesão

ACESSO AO ARTIGO

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