Avaliação dos efeitos do extrato de Chlorella vulgaris sobre a resposta imunologica do tipo celular em camundongos expostos ao chumbo e infectados com Listeria monocytogenes

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

In this work, we investigated the effects of Chlorella vulgaris extract (CVE) in lead-exposed and Listeria monocytogenes infected mice. Experiments were carried out in mice treated simultaneously with CVE and lead acetate for 10 days. At the end of this period, the animals were infected. The capacity of CVE to prevent the myelosuppression induced by lead was demonstrated by the increased numbers of CFU-GM in clonal assays and the restored clonogenic potential of pluripotent stem cells in long-term bone marrow cultures (LTBMC). The extract also stimulated NK cell activity and lymphocyte proliferation in lead-exposed and infected animals. A 34% reduction of blood lead levels was observed when CVE was given to mice simultaneously to lead acetate, suggesting a chelating activity of Chlorella. Experiments in mice lacking a functional IFN-g gene demonstrated that this cytokine is of paramount importance in the protection afforded by CVE. The CVE treatment stimulated the secretion of Th1 cytokines (IL-1, IFN-g e TNF-a) in the infected group, and normal levels of these cytokines were determined in the lead-exposed groups. Likewise, the production of Th2 type cytokines (IL-6 and IL-10) was modulated by the algae extract in all groups studied.

ASSUNTO(S)

imunologia terapia quelante listeriose chumbo celulas hematopoieticas primitivas alga

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