Avaliação do potencial genotóxico do 51Cr2O3, marcador de digestibilidade, por meio da frequência de anormalidades nucleares em tilápias do Nilo (Oreochromis niloticus L.) / The evaluation of genotoxic potential of 51Cr2O3, a digestibility marker, by the frequency of nuclear abnormalities in Nile tilapia (Oreochromis niloticus L.)

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

17/09/2012

RESUMO

Tilapia is the common name of a large number of species of cichlid fish. In aquaculture, this is one of the most profitable fish, since it presents lower feed conversion than other fish. The chromium oxide has been used as a marker for digestibility because it is inert and not alter the biological process of dietary intake. The use of radioactive form of chromium as a marker of digestibility saves time and labor. The aim of this study was to evaluate the genotoxic potential of 51Cr2O3, used as a biological marker in studies of nutrition and physiology, through the frequency of nuclear abnormalities. We used 40 adult, male Nile tilapia (Oreochromis niloticus L.) (01 animal / tank) in a randomized design. The experimental period lasted 21 days, with 14 days to adapt to the installations and receiving encapsulated feed, free of the marker, with 30% crude protein (CP) and 2,800 Kg-1Kcal of digestible energy (DE)/kg. After this period, two capsules containing chromium oxide, 51Cr2O3 (radioactive form), were provided daily (experimental group) and to satiety were administered capsules containing the diet without the marker. We evaluated seven times sampling. The marker was not significantly absorbed by the digestive tract, but the micronucleus test and the frequency of nuclear abnormalities demonstrated that there was significant genotoxic effect on the erythrocytes of juvenile Nile tilapia, exposed to concentrations of 66mCi.g-1 of 51Cr in the form of chromium oxide, for the seven sampling times.

ASSUNTO(S)

micronucleus crômio radiação peixe micronúcleo fish radiation chromium medicina veterinaria

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