Avaliação do comportamento do hidrogel álcool de polivinil (PVA) acetalizado e irradiado como substituto da cartilagem articular implantado em joelhos de ratos / Evaluation behavior polyvinyl alcohol (PVA) hydrogel acetalized and irradiated as substitute of articular cartilage implanted in wistar rat knees

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

21/12/2011

RESUMO

Irradiated and acetalized polyvinyl alcohol (PVA) hydrogel was investigated as a potential material for osteochondral defect repair in articular cartilage. The in vitro cytotoxicity of the material was evaluated with Vero cells and mesenchymal stem cells from bone marrow of Wistar-Kyoto rats. For in vivo analyses, plugs of the material were implanted into defects produced in the knees of male Wistar rats-WH. After follow-up times of 0, 3, 6, 12 and 24 weeks, the samples were analyzed by conventional optical and scanning electron microscopy, X-ray fluorescence spectrometry and submitted to mechanical testing of creep indentation. The in vitro results suggest that the PVA hydrogel did not affect the viability and morphology of any of the cell types studied. The hydrogel affected neither the metabolic activity of both studied cells nor the osteogenic differentiation of the mesenchymal cells. Activity of alkaline phosphatase and mineralized organic matrix formation could be identified. The implanted plugs showed no sign of wear or aspect change. The interface plug/tissue exhibited dense connective tissue and newly formed bone in all groups. It was observed an increase in the creep modulus and in the concentration of calcium and phosphorus in the PVA over time. The performance of the irradiated and acetalized PVA was considered satisfactory for the proposed application.

ASSUNTO(S)

hidrogel Álcool de polivinil defeitos cartilagem articular biomateriais hydrogel polyvinyl alcohol defects articular cartilage biomaterials

Documentos Relacionados