Avaliação da granzima B e do fator de crescimento endotelial vascular em pacientes com neoplasia intra-epitelial escamosa cervical de baixo e de alto grau / Immunohistochemical expression of granzyma B and vascular endothelial growth factor (VEGF) in normal uterine cervix, low and high grade squamous intraepithelial lesions

AUTOR(ES)

Patricia Napoli Belfort Mattos

FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

30/04/2008

RESUMO

Introduction: The granzyme family designates a special group of serinoproteases which are stored fully processed and in the active form in cytoplasmic granules of the cytolytic T lymphocytes cells (CTL). Angiogenesis is the process in which new blood vessels are formed. It is associated with development of high degree intraepithelial neoplasia and invasive carcinoma of the uterine cervix. Objectives: This study objective was to evaluate the immuno-expression of granzyme B and vascular endothelial growing factor (VEGF) in the variants of cervical squamous intraepithelial neoplasia. Methods: Granzyme B immunohistochemical expression was studied in the epithelium, stroma and in both epithelium - stroma of 142 fragments of uterine cervix, being 34 grade I cervical intraepithelial neoplasia (CIN I), 36 grade II cervical intraepithelial neoplasia (CIN II), 33 grade III cervical intraepithelial neoplasia (CIN III) and 39 uterine cervix fragments without abnormalities - control group. Immunoexpression of vascular endothelial growing factor was studied in 160 uterine cervix fragments, being 43 grade I cervical intraepithelial neoplasia (CIN I), 33 grade II cervical intraepithelial neoplasia (CIN II), 31 grade III cervical intraepithelial neoplasia (CIN III) and 53 uterine cervix fragments without abnormalities - control group. Results: In the stroma, immuno-expression of granzyme B in grade I cervical intraepithelial neoplasia was smaller than in grade III cervical intraepithelial neoplasia. High VEGF immune-expression was found in grade III cervical intraepithelial neoplasia while it was low in grade I cervical intraepithelial neoplasia and in control group. Conclusion: The higher the severity of the cervical intraepithelial lesion, the higher the immuno-expression of granzyme B. A progressive increasement in VEGF immune-expression was found in the intense grade, according to the severity of cervical intraepithelial neoplasia.

ASSUNTO(S)

granzima b vegf nic i nic ii nic iii ginecologia e obstetricia

Documentos Relacionados

• Carga viral do papilomavírus humano como fator preditivo de neoplasia intra-epitelial de alto grau em mulheres com células escamosas atípicas ou lesão escamosa intra-epitelial de baixo grau na colpocitologia
• Neoplasia intra-epitelial cervical: diagnóstico e tratamento
• Avaliação da imuno-expressão da granzima B na neoplasia intra-epitelial cervical em mulheres infectadas pelo HIV
• Evaluation of diagnostic procedires of low-grade squamous intraepithelial lesion related to recommende conservative management
• Detecção e genotipagem do papilomavirus humano (HPV) em mulheres com neoplasia intra-epitelial cervical de alto grau