Atividade inibitória da clorofilina (CHLN) na replicacão do poliovírus, rotavírus e herpesvírus, in vitro

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

Chlorophyllin (CHLN) was assayed in poliovirus (PV), bovine herpesvirus (BHV-1) and rotavirus (R) in HEp-2 and MA-104 cell cultures. Three protocols were used: I) Cells were treated with CHLN for 1h and 2h, before infection (prophylactic activity, - 1h and -2h). II) Virus strain was treated with the drug for 1h, before infection (virucide activity). III) CHLN was added to the culture at the moment of infection (therapeutic, zero h), and 1h and 2h after infection, therapeutic 1h and 2h, respectively. Effects were monitored by plaque assay (PFU) and inhibition of fluorescent cell (IFA) for PV and BHV-1, and viral nucleic acid quantification (RNA) and IFA for R. Virucide activity demonstrated: a) Inhibition of R replication in 100% and 70% by RNA and IFA, respectively, representing selectivity indexes (SI) of 33.6 and 22.5, respectively. b) Inhibition of PV and BHV-1 in 62% (SI=22.4) and 66% (SI=22.0) by PFU, respectively. By IFA, inhibition were 57.7% (SI=32.1) and 66% (SI=33.1) for PV and BHV-1, respectively. The time-of-addition study demonstrated: for PV, the highest inhibition, 70% (SI=57.0) was observed under therapeutic protocol 1h (IFA). For BHV-1, the maximum percent of inhibition was found in prophylactic activity -2h (IFA), 77% (SI=18.7). Under therapeutic protocol 0h (PFU), high rate was also found, 66,5% (SI=33,1). For R, the highest percentage was 60% (SI=11.8) in therapeutic protocol 0h (IFA). CHLN activity is possibly on virus particles and/or on virus-receptor sites. We suggest that drug complexation with virion and/or receptors seem to be one of the mechanisms.

ASSUNTO(S)

poliovírus vírus do herpes rotavírus microbiology microbiologia virologia virology poliovirus herpesviridae

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