Apoptosis of human tongue squamous cell carcinoma cell (CAL-27) induced by Lactobacillus sp. A-2 metabolites

AUTOR(ES)

ZHANG, Guoliang, ZHANG, Jie, WANG, Xinyu, YANG, Wenqin, SUN, Zhihui, KUMAR, Chaurasia Nitesh, GUAN, Hong, GUAN, Jian

FONTE

J. Appl. Oral Sci.

DATA DE PUBLICAÇÃO

2014-07

RESUMO

Objective: To study the effect of Lactobacillus sp. A-2 metabolites on viability of CAL-27 cells and apoptosis in CAL-27 cells. Methods: Lactobacillus sp. A-2 metabolites 1 and 2 (LM1 and LM2) were obtained by culturing Lactobacillus sp. A-2 in reconstituted whey medium and whey-inulin medium; the cultured CAL-27 cells were treated with different concentrations of LM1 and LM2 (0, 3, 6, 12, 24, 48 mg/mL) and assayed by methyl thiazolyltetrazolium (MTT) method; morphological changes of apoptotic cell were observed under fluorescence microscopy by acridine orange (Ao) fluorescent staining; flow cytometry method (FCM) and agarose gel electrophoresis were used to detect the apoptosis of CAL-27 cells treated LM1 and LM2. Results: The different concentrations of LM1 and LM2 could restrain the growth of CAL-27 cells, and in a dose-dependent manner; the apoptosis of CAL-27 cells was obviously induced and was time-dependent. Conclusions: Viability of CAL-27 cells was inhibited by Lactobacillus sp. A-2 metabolites; Lactobacillus sp. A-2 metabolites could induce CAL-27 cells apoptosis; study on the bioactive compounds in the Lactobacillus sp. A-2 metabolites and their molecular mechanism is in progress.

Documentos Relacionados

• Giant cell arteritis of the tongue associated with squamous cell carcinoma.
• Clinical-pathological parameters in squamous cell carcinoma of the tongue
• Clinicopathological and immunohistochemical analysis of spindle cell squamous cell carcinoma of the tongue: a rare case
• Squamous cell carcinoma of the tongue in patients older than 45 years
• Frequency of Cervical Nodal Metastasis in Early- Stage Squamous Cell Carcinoma of the Tongue