Anopheles gambiae eicosanoids modulate Plasmodium berghei survival from oocyst to salivary gland invasion

AUTOR(ES)

Ramos, Susana, Custódio, Ana, Silveira, Henrique

FONTE

Mem. Inst. Oswaldo Cruz

DATA DE PUBLICAÇÃO

19/08/2014

RESUMO

Eicosanoids affect the immunity of several pathogen/insect models, but their role on the Anopheles gambiae response to Plasmodium is still unknown. Plasmodium berghei-infected mosquitoes were injected with an eicosanoid biosynthesis inhibitor, indomethacin (IN), or a substrate, arachidonic acid (AA), at day 7 or day 12 post-infection (p.i.). Salivary gland invasion was evaluated by sporozoite counts at day 21 p.i. IN promoted infection upon sporozoite release from oocysts, but inhibited infection when sporozoites were still maturing within the oocysts, as observed by a reduction in the number of sporozoites reaching the salivary glands. AA treatment had the opposite effect. We show for the first time that An. gambiae can modulate parasite survival through eicosanoids by exerting an antagonistic or agonistic effect on the parasite, depending on its stage of development.

Documentos Relacionados

• Anopheles gambiae salivary gland proteins as putative targets for blocking transmission of malaria parasites
• Molecular interactions between Anopheles stephensi midgut cells and Plasmodium berghei: the time bomb theory of ookinete invasion of mosquitoes
• Trapping cDNAs encoding secreted proteins from the salivary glands of the malaria vector Anopheles gambiae
• Immune response of Anopheles gambiae to the early sporogonic stages of the human malaria parasite Plasmodium falciparum
• The role of reactive oxygen species on Plasmodium melanotic encapsulation in Anopheles gambiae