Análise do padrão de metilação do gene Peg3 em diferentes regiões de cérebro de bovinos da raça Nelore / Methylation pattern assay of Peg3 in several regions of Nellore cattle breed brain

AUTOR(ES)

Hélida Regina Magalhães

DATA DE PUBLICAÇÃO

2009

RESUMO

The maternal behavior is essential to survival and development of mammalian offspring. Throughout pregnancy, females receive sensory and hormonal stimuli which promote modifications and prepare the mothers brain to the onset of maternal behavior patterns (for example, by increasing numbers of neurons producing oxytocin in the hypothalamus). Studies have identified the hypothalamus as the main responsible for these changes, but other areas of the brain are also involved in the maternal behavior process. Peg3, an imprinted paternally expressed gene, is known to control maternal behavior in mice. Peg3 knockout females failed in increasing food intake, milk ejection and some maternal activities as placentofagia and nest building. This study aimed to determine the methylation patterns of the differently methylated region of Peg3 (DMR-Peg3) of animals from Nellore cattle breed in several areas of the brain. Samples were collected from the following areas of cattle brain: the frontal, occipital, temporal and parietal cortices, hippocampus and hypothalamus, in a total of 8 animals (4 males and 4 females). The methylation pattern of these samples was analyzed by the protocol COBRA (Combined Bisulfite-Restriction Analysis), which combines DNA modification by sodium bisulfite, PCR amplification and digestion by restriction enzymes. It was found different methylation patterns among the samples. There was a predominance of hypomethylation among male samples, while different patterns were found among the female samples. Variation in the methylation patterns was more markedly observed among samples of the same cerebral region among different animals, then among samples of several regions within an animal. The results suggest that there may be a variation in the imprinting status at a population level, but further assays, with an increased number of samples are needed to verify the statistical significance of this variation.

ASSUNTO(S)

bovinos. cattle. peg3 methylation peg3 imprinting genômico genomic imprinting metilação

Documentos Relacionados

• Análise do padrão de metilação do promotor do gene ATXN3 em pacienetes com a doença de Machado-Joseph
• Seleção assistida por marcadores genéticos de características de carcaça em bovinos da raça Nelore
• Perfil proteômico muscular de bovinos inteiros da raça Nelore
• Padrão de metilação dos genes IGF2 e XIST em ovócitos oriundos de folículos pré-antrais de vacas Nelore (Bos taurus indicus)
• Nutrient requirements of Nellore cattle, purebred and crossbred, of different sexual groups