An optimized one-tube, semi-nested PCR assay for Paracoccidioides brasiliensis detection
AUTOR(ES)
Pitz, Amanda de Faveri, Koishi, Andrea Cristine, Tavares, Eliandro Reis, Andrade, Fabio Goulart de, Loth, Eduardo Alexandre, Gandra, Rinaldo Ferreira, Venancio, Emerson Jose
FONTE
Rev. Soc. Bras. Med. Trop.
DATA DE PUBLICAÇÃO
09/10/2013
RESUMO
Introduction Herein, we report a one-tube, semi-nested-polymerase chain reaction (OTsn-PCR) assay for the detection of Paracoccidioides brasiliensis. Methods We developed the OTsn-PCR assay for the detection of P. brasiliensis in clinical specimens and compared it with other PCR methods. Results The OTsn-PCR assay was positive for all clinical samples, and the detection limit was better or equivalent to the other nested or semi-nested PCR methods for P. brasiliensis detection. Conclusions The OTsn-PCR assay described in this paper has a detection limit similar to other reactions for the molecular detection of P. brasiliensis, but this approach is faster and less prone to contamination than other conventional nested or semi-nested PCR assays.
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