Ambiente de cultivo na propagaÃÃo in vitro de crisÃntemo (Dondranthema grandiflora Tzvelev cv. Rage): caracterÃsticas anatÃmicas e fisiolÃgicas / Environment of culture in the propagation in vitro of Dendranthema grandiflora cv. Rage: anatomical and physiological alterations.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

Plant cultivated for the beauty of its flowers, chrysanthemum has great commercial value for being one of the most important ornamental cultures for plants market . Tissue culture has been used for that plant in commercial propagation. Becouse of high costs in traditional micropropagation tecnics, to the loss during the acclimatizing process and high eletric energy consumption in growth rooms, the objective of this work was to cultivate crisÃntemo in vitro trought an alternative way. The material consists of nodal segment cultivated in vitro, during 60 days. In a first experiment, two different factors had been tested: light (usual growth room - GR, greenhouse with 50% of â GHS and greenhouse without shade - GHWS) and different sucrose concentrations (0, 15 e 30 g.L-1). Number of leafs, sprouts and roots and length of the biggest resulted root the best one was observed 30g.L-1 of sucrose and greenhouse 50% of shadow â GHS. Stomatal density, polar and equatorial diameter in greenhouse without shadow - GHWS and medium aditionated with30g.L-1 of sucrose. In an second experiment it was tested the effect of colorful shading (red and blue) for bottles cultivated in greenhouse without shade - GHWS and growth room - GR. For fitotecnics variables, GR showed more efficience than the too other treatments, however, to the colored screens, better results were obteined GH-red. Best anatomical aspects were gotten in GHWS. The third experiment, there were avaluated two kinds of environment: GHS and GR and two systems of prohibition: ventilated and traditional. For leaf number, sprouts and roots and length of aerial part and roots, the better results had been gotten in GHS and ventilated system of prohibition, however the factors do not interact between itself. Stomatal densities and polar and equatorial diameter, GH with system of ventilated prohibition had the best results. However to mesofilo, GH and the traditional prohibition system showed hight thicknesses. Acording to the results, it is possible to recommend the use of the natural light for in vitro propagation , as well as the use of prohibition systems that allow natural ventilation, providing a reduction in the production costs.

ASSUNTO(S)

luz natural; micropropagaÃÃo; ventilaÃÃo natural; qualidade espectral; aclimatizaÃÃo fisiologia vegetal natural ligth; micropropagation; natural ventilation; spectral quality; acclimatization

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