A espectrometria de massas e as bio-moleculas : relação estrutura/reatividade de peptideos por reações ion/molecula e mobilidade de ions e busca de novos biomarcadores em clinica medica por imageamento quimio-seletivo de tecidos / Mass spectrometry and bio-molecules : structure-reactivity relation of peptides by ion/molecule reactions and ion mobility and search for new biomarkers in clinical medicini for chemo-selective imaging of tissues

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

02/06/2010

RESUMO

The aim of this doctoral project was to study new applications of mass spectrometry (MS) to bio-molecules by using new techniques and recent approaches room pressure, with the goal of obtaining a more rapid and selective identification of an AA in a peptide sequence. Three-dimensional forms of the peptides and their fragment ions formed (a, b and y), were also studied using modern tools of MS, as ion-mobility mass spectrometry (IMMS) and ion / molecule reactions. This study was important because the exact three-dimensional structure of these ions is not yet fully elucidated. A recent technique in mass spectrometry, the chemical imaging by MALDI-MS, was also employed in the search for protein biomarkers for cancer. This technique presents prospects for applications in several areas of great importance as in the medical field since it provides a picture of the chemical constituents of tissues. In this image, cancer can be detected cancer based on the chemical data and not only on the morphology of cells as is normally done today. In this study, we analyzed samples of normal pancreatic tissue, tumor and pancreatitis, and some proteins have been identified and presented themselves as potential biomarkers for this cancer.

ASSUNTO(S)

espectrometria de massas reações ion/molecula peptideos proteinas mass spectrometry ion/molecule reactions peptides protein

ACESSO AO ARTIGO

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