Amebiasis
Mostrando 1-12 de 68 artigos, teses e dissertações.
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1. Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis
Amebiasis is one of the twenty major causes of disease in Mexico; however, the diagnosis is difficult due to limitations of conventional microscopy-based techniques. In this study, we analyzed stool samples using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to differentiate between Entamoeba histolytica (pathogenic) and E. dis
Braz J Med Biol Res. Publicado em: 03/04/2017
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2. Laboratory diagnosis of amebiasis in a sample of students from southeastern Brazil and a comparison of microscopy with enzyme-linked immunosorbent assay for screening of infections with Entamoeba sp.
Introduction: Epidemiological studies on amebiasis have been reassessed since Entamoeba histolytica and E. dispar were first recognized as distinct species. Because the morphological similarity of these species renders microscopic diagnosis unreliable, additional tools are required to discriminate between Entamoeba species. The objectives of our study were
Rev. Soc. Bras. Med. Trop.. Publicado em: 2014-01
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3. Prevalence and characters of Entamoeba histolytica infection in Saudi infants and children admitted with diarrhea at 2 main hospitals at south Jeddah: a re-emerging serious infection with unusual presentation
In this study, Entamoeba histolytica had high prevalence and unusual presentation by affecting high proportion of infants under 1 year; severe clinical manifestations, and laboratory findings that were known to be usually encountered in invasive amebiasis as significant leukocytosis for age, neutrophilic leukocytosis for age, and positive C-reactive protein
Braz J Infect Dis. Publicado em: 2013-02
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4. Hepato-pulmonary amebiasis: a case report
Infections with Entamoeba histolytica are seen worldwide and are more prevalent in the tropics. About 90% of infections are asymptomatic, and the remaining 10% produce a spectrum of clinical syndromes, ranging from dysentery to abscesses of the liver or other organs. Extra-intestinal infection by E. histolytica most often involves liver. Pleuro-pulmonary inv
Brazilian Journal of Infectious Diseases. Publicado em: 2010-08
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5. Comparison of multiplex-PCR and antigen detection for differential diagnosis of Entamoeba histolytica
Amebiasis is an infection caused by Entamoeba histolytica. However, differentiation between E. histolytica and Entamoeba dispar, which are morphologically identical species, is essential for treatment decision, precaution of the invasive disease and public health. The purpose of the present study was to evaluate a Multiplex -PCR for detection and differentia
Brazilian Journal of Infectious Diseases. Publicado em: 2007-06
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6. Evaluation of the Therapeutical Effectiveness of the Piperazine Hexahidratada associated with the Fluid Extract of Rhamus Purshiana in the treatment of the Worn Infections / Estudo comparativo, randomizado para avaliar a eficÃcia terapÃutica da piperazina hexahidratada com extrato fluido de rhamnus purshiana no tratamento da ascaridÃase
The efficacy and the therapeutic security of a manufactured piperazina associated with the extract fluid of Rhamnus purshiana (DM IndÃstria FarmacÃutica) used for deworming patients in CearÃ, Brazil, were tested against the product composed by piperazina without any association (DM IndÃstria FarmacÃutica). A prospective, randomized, controlled and doubl
Publicado em: 2007
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7. Intestinal parasitic infections and eosinophilia in an human immunedeficiency virus positive population in Honduras
The occurrence of intestinal parasites, their regional distribution and their relations to eosinophilia were studied in 133 human immunodeficiency virus (HIV) positive individuals from Honduras. After signing an informed consent, participants answered a socio-demographic and risk factor questionnaire, a complete physical examination, medical history, and a s
Memórias do Instituto Oswaldo Cruz. Publicado em: 2004-11
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8. Hepatic amebiasis
Amebiasis can be considered the most aggressive disease of the human intestine, responsible in its invasive form for clinical syndromes, ranging from the classic dysentery of acute colitis to extra-intestinal disease, with emphasis on hepatic amebiasis, unsuitably named amebic liver abscess. Found worldwide, with a high incidence in India, tropical regions o
Brazilian Journal of Infectious Diseases. Publicado em: 2003-04
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9. Salpingitis due to Entamoeba histolytica
We describe the pathology of a unique case of Fallopian tube amebiasis, associated with hydrosalpinx, in a 21-year-old woman. She complained of lower abdominal pain, had a foul-smelling green vaginal discharge and fever during one week. There was a discrete increase in body temperature and a painful abdominal palpation at the lower right side, with signs of
Brazilian Journal of Infectious Diseases. Publicado em: 2002-04
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10. Entamoeba histolytica: efficacy of microscopic, cultural, and serological techniques for laboratory diagnosis.
Of 110 subjects with clinical evidence of amebiasis, 15 (14%) were shown to be infected with Entamoeba histolytica. Microscopic examination of stool specimens rendered a diagnosis in all eight cases of localized intestinal infection, but in only one of seven patients with invasive amebiasis. Culture was concomitantly diagnostic in six patients intestinal ame
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11. Recombinant antigen-based avidin-biotin microtiter enzyme-linked immunosorbent assay for serodiagnosis of invasive amebiasis.
An immunoscreening approach was used to isolate a strongly positive cDNA clone from an Entamoeba histolytica HK-9 cDNA expression library in the phage vector lambda ZAP-II. The 1.85-kb cDNA insert was found to be truncated and encoded the cysteine-rich, immunodominant domain of the antigenic 170-kDa subunit of the amebal galactose-N-acetylgalactosamine bindi
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12. Sensitive and specific serodiagnosis of invasive amebiasis by using a recombinant surface protein of pathogenic Entamoeba histolytica.
A recombinantly expressed protein, recEh-P1, representing part of an immunodominant surface antigen of pathogenic Entamoeba histolytica, was used for serodiagnosis of invasive amebiasis. Expression was performed under the control of a T7-RNA promoter by using a modified procaryotic expression vector, designated pHisT7. This vector allowed high-yield expressi