Aedes Albopictus Cells
Mostrando 1-12 de 62 artigos, teses e dissertações.
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1. Detection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virus
BACKGROUND The Zika virus (ZIKV) epidemics that affected South America in 2016 raised several research questions and prompted an increase in studies in the field. The transient and low viraemia observed in the course of ZIKV infection is a challenge for viral isolation from patient serum, which leads to many laboratories around the world sharing viral stra
Mem. Inst. Oswaldo Cruz. Publicado em: 07/02/2019
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2. Ecological aspects of mosquitoes (Diptera: Culicidae) in the gallery forest of Brasilia National Park, Brazil, with an emphasis on potential vectors of yellow fever
Introduction We analyzed the vertical and monthly distributions of culicid species in the gallery forest of Brasília National Park, with an emphasis on the potential vectors of yellow fever (YF). Methods Between September 2010 and August 2011, mosquitoes were captured on the ground and in the canopy of the forest for five consecutive days per month, fro
Rev. Soc. Bras. Med. Trop.. Publicado em: 15/10/2013
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3. A comparative study of fat body morphology in five mosquito species
The insect fat body plays major roles in the intermediary metabolism, in the storage and transport of haemolymph compounds and in the innate immunity. Here, the overall structure of the fat body of five species of mosquitoes (Aedes albopictus, Aedes fluviatilis, Culex quinquefasciatus, Anopheles aquasalis and Anopheles darlingi) was compared through light, s
Mem. Inst. Oswaldo Cruz. Publicado em: 2011-09
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4. Comparison of dengue infection in human mononuclear leukocytes with mosquito C6/36 and mammalian Vero cells using flow cytometry to detect virus antigen
Fluorescent activated cell sorter (FACS) analysis is useful for the detection of cellular surface antigens and intracellular proteins. We used this methodology in order to detect and quantify dengue antigens in highly susceptible cells such as clone C6/36 (Aedes albopictus) and Vero cells (green monkey kidney). Additionally, we analyzed the infection in vitr
Memórias do Instituto Oswaldo Cruz. Publicado em: 2000-08
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5. Antiviral activity released from Aedes albopictus cells persistently infected with Semliki forest virus.
Aedes albopictus (mosquito) cells persistently infected with Semliki Forest virus released an agent which inhibited virus production by A. albopictus cells infected with homologous virus. Inhibition of virus production was accompanied by a marked reduction in the synthesis of viral RNA and viral proteins. Expression of the antiviral effect was prevented by p
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6. Singh’s Aedes albopictus Cell Cultures as Helper Cells for the Adaptation of Obodhiang and Kotonkan Viruses of the Rabies Serogroup to Some Vertebrate Cell Cultures
Multiplication of rabies serogroup viruses, Obodhiang and kotonkan, was induced in vertebrate cell cultures by using Singh’s Aedes albopictus cell cultures as „helper cells.”
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7. Homologous Viral Interference in Aedes albopictus Cultures Chronically Infected with Sindbis Virus
Complete homologous interference is demonstrated in cultures of Aedes albopictus cells chronically infected with Sindbis virus. The interference occurred before there was any detectable RNA synthesis by the superinfecting virus.
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8. Failure of defective interfering particles of Sindbis virus produced in BHK or chicken cells to affect viral replication in Aedes albopictus cells.
Whereas defective interfering particles of Sindbis virus are readily produced in BHK-21 cells or chicken embryo fibroblasts by the techniques of serial undiluted passage, similar methods failed to generate such particles in Aedes albopictus cell cultures. In addition, Sindbis virus stocks produced in BHK-21 cells or chicken embryo fibroblasts and which conta
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9. Selective disappearance of two secreted host proteins in the course of Semliki Forest virus infection of Aedes albopictus cells.
One secreted host protein of molecular weight 54,000 (SP 54) disappeared (from 24 to 48 h after infection) in Semliki Forest virus-infected Aedes albopictus cell clone C6/36 grown in both Mitsuhashi-Maramorosch basal medium and tissue culture medium 199 and reappeared when cells went into the permanently infected state. C6/36 is a high virus producer showing
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10. Suppression of RNA synthesis by a specific antiviral activity in Sindbis virus-infected Aedes albopictus cells.
An antiviral protein is released by mosquito cells persistently infected with Sindbis virus. Differences in both sensitivity to and production of this virus-specific activity were apparent in three independently produced Aedes albopictus cell lines. This activity inhibits total viral RNA synthesis in a time-dependent manner. The antiviral effect is maximally
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11. Characteristics of Sindbis virus temperature-sensitive mutants in cultured BHK-21 and Aedes albopictus (Mosquito) cells.
A number of the temperature-sensitive mutants of Sindbis virus originally isolated and characterized by Burge and Pfefferkorn (1966, 1968) were reexamined for their abilities to grow and complement one another in cultured BHK-21 and Aedes albopictus (mosquito) cells. The response of the mutants to conditions of high and low temperature was similar in culture
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12. Origin of the actinomycin D insensitive RNA species in Aedes albopictus cells.
In the presence of actinomycin D or a combination of actinomycin D and either camptothecin or alpha-amanatin. Aedes albopictus cells synthesize a variety of single stranded RNA species. These actinomycin D resistant species are ethidium bromide sensitive and they are present in the cell cytoplasm in an RNase resistant structure which has the sedimentation an